Steven T. Boyce, Ph.D.

Depts of Surgery and Biomedical Engineering
University of Cincinnati
P.O. Box 670558
Cincinnati, Ohio 45267-0558
E-mail: boycest@uc.edu
Office: (513)558-7707

Depts of Tissue Engineering & Research
Shriners Burns Hospital
3229 Burnet Avenue
Cincinnati, Ohio 45229-3095
E-mail: stboyce@shrinenet.org
Office: (513)872-6080
FAX: (513)872-6107

PROFESSIONAL AND
RESEARCH EXPERIENCE:

Shriners Burns Hospital Cincinnati, Ohio
1997 - PRES Director, Skin Substitute Laboratories; Department of Tissue Engineering
1989 - PRES Senior Investigator; Research Department

University of Cincinnati College of Medicine Cincinnati, Ohio
2002 - PRES Professor with tenure; Department of Surgery
1999 - 2002 Associate Professor with tenure; Department of Surgery
1996 - 1999 Associate Professor; Department of Surgery
1989 - 1997 Director, Electron Microscopy Laboratories; Department of Surgery
1995 - 1996 Research Associate Professor; Department of Surgery
1989 - 1995 Research Assistant Professor; Department of Surgery
1990 - 1995 Adjunct Assistant Professor; Department of Anatomy and Cell Biology

University of Cincinnati Colleges of Medicine & Engineering Cincinnati, Ohio
2002 - 2004 Adjunct Associate Professor, Department of Biomedical Engineering

US Food and Drug Administration Rockville, Maryland
1993 - 1997 Voting Member; General and Plastic Surgery Devices Panel of the Medical Devices Advisory Committee to the Center for Devices and Radiological Health
1998 - 2005 Ad Hoc Consultant, General and Plastic Surgery Devices Panel of the Medical Devices Advisory Committee to the Center for Devices and Radiological Health

University of California San Diego Medical Center San Diego, California
1985 ﷓ 1988 Assistant Adjunct Professor; Department of Surgery

University of Colorado Health Sciences Center Denver, Colorado
1983 ﷓ 1984 Research Associate; Department of Surgery

University of Colorado Boulder and Denver, Colorado
1981 ﷓ 1984 Pre-doctoral Fellow; Department of Molecular Cellular & Developmental Biology
1975 ﷓ 1981 University Researcher II, Laboratory Technician II and I


EDUCATION:

Ph.D., 1984 B.A., 1974
University of Colorado University of Colorado
Boulder, Colorado Boulder, Colorado
Molecular, Cellular, Biological Sciences
and Developmental Biology

MEMBERSHIPS AND OFFICES IN ACADEMIC SOCIETIES:

1980- Society for In Vitro Biology (formerly the Tissue Culture Association);
1994-95 President, Ohio Valley Branch
1994-95 Liaison from SIVB to American Assn of Tissue Banks
1998 Established Investigator Award
1983- American Burn Association;
1991-94, member of the At-Large Membership Committee
1996-99, member of the Research Committee
1999 Clinical Research Award
1984- Society for Investigative Dermatology
1985- Society for Biomaterials
1989- American Society for Cell Biology
1991- American Association of Tissue Banks;
1993-95, member of the Science and Technology Committee
1999-2002, member of the Medical Advisory Committee
2001-2004, liaison to the American Society for Testing and Materials
1992-2003 Microscopy Society of America;
1993 Treasurer, national meeting Local Arrangements Committee
1994- Wound Healing Society
1997- American Society for Testing and Materials
1998-2001 task group F04.43.05 on tissue engineered skin products
1998-2001 vice chair of subcommittee on cells and processing
2005- Tissue Engineering Society International

TECHNICAL EXPERTISE:

Development of serum﷓free isolation and primary culture of human cells
Development of defined clonal culture systems for human cells
Biochemical and biophysical procedures for preparation of dermal skin substitutes
Biocompatibility of implantable materials
Fabrication, anatomy and physiology of cultured skin substitutes
Clinical investigations of wound healing with cultured skin substitutes
Quantitative assessment of wounds with biophysical instrumentation
Light microscope histology, immunohistochemistry and in situ hybridization
High voltage, scanning, and transmission electron microscopy
Autoradiography and radio-isotope assays
Image analysis
Polyacrylamide gel electrophoresis, and western blot analysis
Protein purification
Quantitative enzyme biochemistry
Cell cycle analysis by flow cytometry
Antibody and biotin﷓avidin types of molecular labeling


PATENTS:

1. Boyce ST and RG Ham. 1986. US Patent 4,673,649, "Process and defined medium for growth of human epidermal keratinocyte cells." Owner: University of Colorado; Assignee: University Patents, Inc.

2. Boyce ST and RG Ham. 1990. US Patent 4,940,666, Continuation in part of US Patent 4,673,649, "Process and defined medium for growth of human epidermal keratinocyte cells." Owner: University of Colorado; Assignee: University Patents, Inc.

3. Boyce ST. 1993. European Patent 363,400, "Method and apparatus for preparing composite skin replacement". Owner: University of California.

4. Boyce ST. 1993. US Patent 5,273,900, "Method and apparatus for preparing composite skin replacement." Owner: University of California.

5. Boyce ST. 1998. US Patent 5,711,172, AApparatus for preparing composite skin replacement@. Owner: University of California.

6. Boyce ST. 1999. US Patent 5,976,878, AMethod and apparatus for preparing composite skin replacement@. Owner: University of California.

7. Boyce ST. 2005. US Patent 6,905,105, AApparatus for fabricating a biocompatible matrix@. Owners: University of Cincinnati and Shriners Hospitals for Children.

8. Boyce ST. 2002. US patent application, publication #US2003/0170892, AA surgical device for replacement of skin@. Owners: University of Cincinnati and Shriners Hospitals for Children.

AWARDS and HONORS:

1. Macy Scholarship at Columbia University College of Physicians and Surgeons; Physiology and Pathology for Cell Biologists; June-August, 1982.

2. Graduate Research Assistantship; US Army Research and Development Command; March﷓ October, 1983.

3. Faculty Achievement Award; University of Cincinnati; November, 1996.

4. Health Care Heroes - Innovator Award; Cincinnati Business Courier; Cincinnati, OH, February, 1998.

5. Established Investigator Award; Society for In Vitro Biology; Las Vegas, NV; June, 1998.

6. Clinical Research Award; American Burn Association; Orlando, FL; March, 1999.

7. Seventh most cited first-author in the dermatology literature, 1982-1996; Arch Dermatol 135:299-302, March, 1999.


INVITED PRESENTATIONS:

1. "Biology of the keratinocyte in vitro"; Cutaneous Biology Foundation; October, 1982.

2. "Calcium﷓regulated proliferation and differentiation of normal human epidermal keratinocytes in vitro"; Stanford University School of Medicine, Department of Dermatology, February, 1983.

3. "First sino﷓american conference on burn injuries"; Joint Sino﷓American Burn Associations; Panel moderator on skin replacements and skin substitutions; Chongqing, China; October, 1985.

4. "Cultured keratinocytes and synthetic skin"; 17th World Congress of Dermatology; Berlin, Germany; May, 1987.

5. "Culture of human epidermal keratinocytes on synthetic biomembranes"; Center for International Research of Dermatology (CIRD); Valbonne, France; June, 1987.

6. "Progress in in vitro toxicology"; Center for Alternatives to Animal Testing; Johns Hopkins University, Baltimore, Maryland; November, 1987.

7. "Tissue engineering of human skin"; UCLA Symposia on Molecular and Cellular Biology; Lake Tahoe, California; February, 1988.

8. "Banking of cultured skin substitutes: Current Status and Future Prospects"; American Association of Tissue Banks; San Diego, California; August, 1988.

9. "Wound healing and wound management"; International Society for Burn Injuries; Chicago, Illinois; October, 1988.

10. "Serum-free culture of human epidermal keratinocytes and applications to skin substitutes"; Second Bioscience Conference, Tokyo Metropolitan University, Tokyo, Japan; November, 1988.

11. Citizen Ambassador Program; People to People Bioprosthetics Delegation to the People's Republic of China; Beijing, Shanghai, Chengdu, Hong Kong, China; May, 1989.

12. "Combined bio-synthetic dermis and cultured human skin cells for wound closure"; International Symposium on Artificial Skin, Koken BioScience Institute; Tokyo, Japan; June, 1989.

13. "Keratinocyte growth"; 51st Annual Meeting of the Society for Investigative Dermatology; Washington, D.C.; May, 1990.

14. "Reconstructed skin from cultured human cells and biopolymers"; Sixth Intl Workshop on In Vitro Toxicology; Seillac, France; October, 1990.

15. "Biopolymer materials for culture and transplantation of human cells"; Department of Pharmacology; Hopital Eduord Herriot; Lyon, France; October, 1990.

16. "Transplantation of cultured skin substitutes"; Department of Dermatology, Boston University School of Medicine; Boston, Massachusetts; September, 1990.

17. "Skin wound closure with cultured human cells, biopolymers and growth factors"; Department of Biology, Case Western Reserve University; Cleveland, Ohio; December, 1990.

18. "Pre-clinical and clinical studies with cultured skin substitutes"; Department of Research, American Red Cross; Bethesda, Maryland; June, 1991.

19. "Skin as a biological membrane"; Department of Chemical Engineering, University of Cincinnati Center of Excellence of Membrane Technology Seminars; November, 1991.

20. "Artificial skin"; American Burn Association, Breakfast Session Co-Moderator; Salt Lake City, Utah; April, 1992.

21. "Composite grafts of cultured human skin cells and biopolymers for burns"; Department of Biological Sciences; Wright State University; Dayton, Ohio; April, 1992.

22. "Methodologies and in vitro applications for organotypic skin culture models"; Organogenesis, Inc.; New York, New York; June, 1992.

23. "Criteria and assays for selection of topical antimicrobial agents for use with cultured skin for burns"; Fernstrom Symposium; Lund, Sweden; June, 1992.

24. "Allogeneic skin and cultured cells for permanent closure of skin wounds"; American Association of Tissue Banks; San Diego, California; August, 1992.

25. "Current usage/trends/research in skin grafts"; South Eastern Organ Procurement Foundation; Orlando, Florida; January, 1993.

26. "Frontiers in burn wound care"; American Burn Association, Ad Hoc Moderator; Cincinnati, Ohio; March, 1993.

27. "Regeneration of skin pigmentation after transplantation of cultured melanocytes to athymic mice"; L'Oreal Cosmetics; Paris, France; August, 1993.

28. "Skin substitutes using cultured cells and biopolymers"; Department of Dermatology, University of Utah; Salt Lake City, Utah; August, 1993.

29. "Wound closure with cultured cells and biopolymers"; Department of Surgery; Indiana University; Indianapolis, Indiana; September, 1993.

30. "Regeneration of epidermal barrier and pigmentation in cultured skin for wound treatment"; Genzyme Corporation, Framingham, Massachusetts; October, 1993.

31. "Artificial skin"; American Burn Association, Breakfast Session Co-Moderator; Orlando, Florida; April, 1994.

32. "Cultured epithelial autografts and the burn wound"; American Burn Association, Correlative Session Co-moderator; Orlando, Florida; April, 1994.

33. "Treatment of chronic wounds with cultured skin substitutes"; University of Miami, Miami, Florida; April, 1994.

34. "Principles and practices for wound treatment with cultured skin substitutes"; Laboratory for Experimental Organogenesis, Hospital St. Sacrement; Quebec City, Canada; May, 1994.

35. "Biological, technical and regulatory considerations for banking of cultured skin"; American Association of Tissue Banks; San Francisco, California; August, 1994.

36. "Treatment of skin wounds with cultured cells and biopolymers"; Dutch Society for Biomaterials; Lunteren, The Netherlands; October, 1994.

37. "Substrate choices for cultured tissue"; American Burn Association, Breakfast Session Co-moderator; Albuquerque, New Mexico; April, 1995.

38. "Burn wound biology"; American Burn Association; Session Co-moderator; Albuquerque, New Mexico; April, 1995.

39. "Skin replacement strategies: where have we been, and where are we going?"; American Burn Association, Plenary Session; Albuquerque, New Mexico; April, 1995.

40. "Closure of full-thickness burns with grafts of cultured skin cells and biopolymers"; Wound Healing Society, Plenary Session; Minneapolis, Minnesota; April, 1995.

41. AQuantification of wound healing in burns@; US Food and Drug Administration, Intercenter Focus Group on Wound Healing; Gaithersburg, Maryland; July, 1995.

42. ACompliance of cultured allogeneic skin cells with regulatory requirements for safe use of cadaveric human skin@; Skin Council of the American Association of Tissue Banks; Atlanta, Georgia; September, 1995.

43. APrinciples for fabrication and applications of cultured skin substitutes@; New Technologies Workshop; Microbiological Associates; Bethesda, Maryland; September, 1995.

44. ABiology and surgery of cultured skin substitutes for wound closure@; Department of Surgery, University of Alberta; Edmonton, Alberta, Canada; October, 1995.

45. "Grafts of cultured cells and biopolymers for treatment of acute and chronic skin wounds"; 7th Polymer Symposium; Kyoto University; Kyoto, Japan; November, 1995.

46. AFactors to consider for culture and transplantation of skin cells@; US FDA Part 15 Public Hearing on Autologous Cells; Gaithersburg, Maryland; November, 1995.

47. ARepair of acute and chronic skin wounds with cultured cells and biopolymers@; Dept of Biomedical Engineering; Univ of Virginia; Charlottesville, Virginia; December, 1995.

48. AEngineering of skin substitutes for wound closure@; Clemson University, Department of Bioengineering; Clemson University, Clemson, SC; January, 1996.

49. AWound repair with cultured cells and biopolymers@; 3M Life Sciences Technical Forum; Minneapolis, MN; January, 1996.

50. ASkin replacements@; American Burn Association, Session Co-moderator; Nashville, TN; March, 1996.

51. ADialogue with FDA: definitions and assessments of healing in burn wounds@; Federal Issues Committee of the American Burn Association, Panelist; Nashville, TN; March, 1996.

52. AComposite skin replacement@; Wound Healing Society, Plenary Session; Boston, MA; May, 1996.

53. AQuantitative assessment of wounds with non-invasive instrumentation@; Wound Healing Society, Instructional Workshop; Boston, MA; May, 1996.

54. ATreatment of burns and chronic wounds with cultured skin substitutes@; Oregon Health Sciences University; Portland, OR; June, 1996.

55. ASkin substitutes for burn care@, in Burn Care: State of the Art, Current Therapy and Future Trends; Shriners Burns Institute; Cincinnati, OH; November, 1996.

56. APrinciples and practices for engineering of skin substitutes@; FDA course on Tissue Engineering; Bethesda, MD; February, 1997.

57. ACultured skin substitutes in burn care@; American Burn Association, Graduate Course on Wound Healing; New York, NY; March, 1997.

58. AHypertrophic scar@; American Burn Association, correlative session co-moderator; New York, NY; March, 1997.

59. ACultured skin substitutes grafted to athymic mice@; Perspectives in Percutaneous Penetration; La Grande Motte, Languedoc, France; April, 1997.

60. ACytokines in the skin@; Proctor & Gamble Company, scientific seminar; Cincinnati, OH; November, 1997.

61. ATopical scar remodeling@; American Burn Association, breakfast session co-moderator; Chicago, IL; March, 1998.

62. AExpression by cultured skin substitutes of molecular mediators and tissue phenotypes@: Plenary presentation. Eighth annual meeting of the Wound Healing Society; Salt Lake City, UT; June, 1998.

63. AStandards for engineering of skin substitutes@; 12th International Symposium on Bioengineering and the Skin; keynote address; Boston, MA; June, 1998.

64. AWhy and how to use cultured skin for burns@; Ohio Burn Team Task Force annual symposium; Cincinnati, OH; October, 1998.

65. AThe normal biology of human skin and cutaneous wound healing@; Society for In Vitro Biology Session in Depth; New Orleans, LA; June, 1999.

66. ATissue engineering with cultured cells and biopolymers: a clinical reality@; Society for In Vitro Biology; Session Chairman; New Orleans, LA; June, 1999.

67. AGrafts with autologous, homologous, heterologous, and artificial skin@; Wound Healing Society, plenary session; Minneapolis, MN; June, 1999.

68. ACultured skin substitutes for closure of cutaneous wounds@; International Business Communications; Boston, MA; June, 1999.

69. AQuantitative assessment of tissue-engineered skin for burns@; American Burn Association, Bioengineering Special Interest Group; Las Vegas, NV; March, 2000.

70. ARecent advances in wound coverage; weighing the options@; American Burn Association, breakfast session co-moderator; Las Vegas, NV; March, 2000.

71. ACultured skin substitutes for closure of full-thickness skin wounds@; First L=Oreal International Symposium on the Science and Technology of Skin; Lyon, France; October, 2000.

72. ABiological skin substitutes: are they worth the cost?@; American Burn Association; Breakfast session; Boston, MA; April, 2001.

73. ASkin substitutes and wound treatment@; Wound Healing Society, session moderator; Albuquerque, NM; May, 2001.

74. AEngineering of human skin@; Swiss Federal Institute of Technology; Department of Chemical Engineering; Zurich, Switzerland; September, 2001.

75. AHuman allograft skin as a source of cells for cultured skin substitutes@; American Association of Tissue Banks; Cincinnati, OH; November, 2001.

76. ACutaneous wound repair with cultured cells and biopolymers@; Department of Biology; University of Dayton; November, 2001.

77. AEpithelial biology and skin repair@; Timberline Symposium on Epithelial Cell Biology; Portland, OR; February, 2002.

78. AArtificial skin: It=s impact on scar formation@; American Burn Association; postgraduate course; Chicago, IL; April, 2002.

79. ATranslational research to improve burn care@; American Burn Association; plenary presentation; Chicago, IL; April, 2002.

80. AInnovations in burn care@; American Burn Association; Chicago, IL; April, 2002.

81. AWound care: skin substitutes/basic science@; American Burn Association; session moderator, Chicago, IL; April, 2002.

82. ABurn wound closure with cultured skin substitutes@; British Burn Association; Birmingham, UK; May, 2002.

83. ACulture and transplantation of skin or cornea epithelium@; Cell Therapies and Tissue Engineering; Case Western Reserve University; Cleveland, OH; June, 2002.

84. ACombination products containing live cellular components@; US Food and Drug Administration; Rockville, MD; June, 2002.

85. ABasic science of skin replacement - state of the art@; International Society for Burn Injuries; Seattle, WA; August, 2002.

86. ATissue engineering@; International Society for Burn Injuries; session moderator; Seattle, WA; August, 2002.

87. AAging factors in wound repair with cultured skin substitutes@; L=Oreal Symposium on Skin Aging; Cleveland, OH; September, 2002.

88. ABiophysical properties of cultured skin substitutes@; International Society for Bioengineering of the Skin; Baltimore, MD; October, 2002.

89. AWound Management@; American Burn Association; session moderator; Miami, FL; April, 2003.

90. ACellular Dermato-therapeutics@; Society for Investigative Dermatology; Miami, FL; May, 2003.

91. “Barrier formation in cultured skin substitutes”; Gordon Conference on Mammalian Skin Barrier; Providence, RI; August, 2003.

92. “Tissue engineering for reconstruction”; American Burn Association; breakfast session moderator; Vancouver, British Columbia, Canada; April, 2004.

93. “Reconstruction”; American Burn Association; correlative session moderator; Vancouver, British Columbia, Canada; April, 2004.

94. “Skin repair with cultured cells and biopolymers”; Keynote address; Australia-New Zealand Burn Association (ANZBA) meeting; Adelaide, Australia; September, 2004.

95. “Tissue regeneration for wound closure”; Australia-New Zealand Burn Association (ANZBA) meeting; Adelaide, Australia; September, 2004

96. “Science and technology of skin engineering”; L’Oreal Recherche; program committee; Lausanne, Switzerland; October, 2004.

97. “Advances in Pediatric Burn Care”; American Academy of Pediatrics; Washington, DC; October, 2005.

98. “Stem cells in wound healing”; British Society for Cell Biology; London, UK; March, 2006.

99. “Tissue engineering for burn wounds”; Wound Healing Society; Tucson, AZ; May, 2006

INVESTIGATIONAL DEVICE EXEMPTIONS (IDE) FILED WITH FDA:

1. IDE G900205. PI, ST Boyce. AAutologous Cultured Skin Substitute@. Feasibility of safety for transplantation of autologous skin cells attached to a degradable polymer matrix for treatment of excised, full-thickness burns. Opened, April 1990; closed, February, 1999.

2. IDE G960013. PI, ST Boyce. AAllogeneic Cultured Skin Substitute@. Feasibility for transplantation of allogeneic skin cells attached to a degradable polymer matrix for treatment of debrided, full-thickness chronic wounds from venous stasis, diabetic or decubitus etiologies. Opened, April, 1996.

3. IDE G980023. PI, ST Boyce. AAutologous Cultured Skin Substitute@. Pre-pivotal study of safety and efficacy for transplantation of autologous skin cells attached to a degradable polymer matrix for treatment of excised, full-thickness burns. Opened, February, 1998.

BOOK CHAPTERS:

1. Boyce ST and RG Ham. 1986. Normal human epidermal keratinocytes. In, "In Vitro Models for Cancer Research; Vol 3, Carcinomas of the Mammary Gland, Uterus and Skin"; editors, MM Webber and LI Sekely; CRC Press, Boca Raton, Florida, pp. 245﷓274.

2. Boyce ST, JF Hansbrough and DA Norris. 1988. Cellular responses of cultured human epidermal keratinocytes as models of toxicity to human skin. In, "Alternative Methods in Toxicology; Vol. 6, Progress in In Vitro Toxicology." (A Goldberg, editor); Leibert Publishers, New York, New York, pp. 27-37.

3. Boyce ST, TJ Foreman and JF Hansbrough. 1988. Functional wound closure with dermal﷓epidermal skin substitutes prepared in vitro. In, "Tissue Engineering"; (R Skalak and C Fox, editors); UCLA Symposia on Molecular and Cellular Biology, Vol. 107:81-86; Alan R Liss, Inc., NY, NY.

4. Boyce ST and IA Holder. 1995. Criteria and assays for selection of topical antimicrobial agents for use with cultured skin for burns. In, "Molecular Pathogenesis of Surgical Infections@; eds, IA Holder and T Wadstrom; pp. 96-102.

5. Boyce ST. 1996. Skin repair with cultured cells and biopolymers. Chapter 15; In, "Human Biomedical Applications"; editor, DL Wise; The Humana Press, Inc.; Totowa, NJ, pp 347-377.

6. Boyce ST. 1997. Cultured skin for wound closure. Chapter 4; In, ASkin Substitute Production by Tissue Engineering: Clinical and Fundamental Applications@; editor, M Rouahbia; RG Landes Company, Austin, TX; pp. 75-102.

7. Boyce ST. 1998. Methods for serum-free culture of keratinocytes and transplantation of collagen-GAG based composite grafts. Methods in Molecular Medicine, Volume 18: AMethods in Tissue Engineering@, Chapter 28; editors, M Yarmush, and JR Morgan; Humana Press, Inc.; Totowa, NJ; pp. 365-389.

8. Supp DM and ST Boyce. 2001. Genetic modification of cultured skin substitutes. In, ACultured Human Keratinocytes and Tissue Engineered Skin Substitutes@; editors, RE Horch, AM Munster, BM Achauer. pp. 60-71.

9. Boyce, ST. 2002. Regulatory issues and standardization. Chapter 1; In, AMethods of Tissue Engineering@; editors, A Atala and R Lanza. Academic Press, San Diego, CA. pp. 3-17.

10. Supp DM and ST Boyce. 2003. Cutaneous gene therapy with cultured skin substitutes. In, "Human Biomedical Applications"; editor, DL Wise; Marcel Dekker, Inc.; New York, NY. pp. 155-172.

11. ST Boyce and DM Supp. 2005. Skin substitutes: theoretical and developmental considerations. In, AFunctional and Aesthetic Reconstruction of Burn Patients@; editor, RL McCauley; Marcel Dekker, Inc.; New York, NY. pp.113-128.

12. DM Supp and Boyce ST. 2005. Bioengineering of human skin substitutes. In, “Biomedical Engineering Handbook”; editors, JP Fisher and AG Mikos; CRC Press, Inc.; Boca Raton, FL. In press.

13. Supp DM and ST Boyce. 2005. Engineered skin substitutes: practices and potentials. In, “Clinical Applications of Tissue Engineering”, editor, D Andreassi; Mary Ann Liebert, Baltimore, MD. In press.

INVESTIGATIVE and INVITED ARTICLES:

1. Bettger WJ, ST Boyce, BJ Walthall and RG Ham. 1981. Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid﷓enriched synthetic medium supplemented with epidermal growth factor, insulin, and dexamethasone. Proc Natl Acad Sci USA 78:5588﷓5592.

2. Boyce ST and RG Ham. 1983. Calcium﷓regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum free serial culture. J Invest Dermatol 81, supplement 1:33S﷓40S.

3. Hansbrough JF and ST Boyce. 1984. What criteria should be used for designing artificial skin replacements and how well do the current materials meet these criteria. J. Trauma 24(9), supplement, S31﷓S35.

4. LeFeber WP, DA Norris, SR Ryan, JC Huff, LA Lee, M Kubo, ST Boyce, BL Kotzin and WL Weston. 1984. Ultraviolet light induces binding of antibodies to selected nuclear antigens on cultured human keratinocytes. J Clin Invest 74:1545﷓1551.

5. Boyce ST and RG Ham. 1985. Cultivation, frozen storage, and clonal growth of normal human epidermal keratinocytes in serum﷓free media. J Tiss Cult Meth 9(2):83﷓93.

6. Norris DA, SR Ryan, RM Kissinger, KA Fritz and ST Boyce. 1985. Systematic comparison of antibody﷓mediated mechanisms of keratinocyte lysis in vitro. J Immunol 135(2): 1073﷓1079.

7. Wikner NE, JC Huff, DA Norris, ST Boyce, M Cary, M Kissinger and WL Weston. 1986. Study of HLA﷓DR synthesis in cultured human keratinocytes. J Invest Dermatol 87:559﷓564.

8. Boyce ST. 1987. Cultured keratinocytes and synthetic skin. Proceedings of the 17th World Congress on Dermatology; eds., CE Orfanos, R Stadler, H Gollnick; Springer﷓Verlag, Heidelberg, Germany, pp 729-733.

9. Boyce ST and JF Hansbrough. 1988. Biologic attachment, growth, and differentiation of cultured human epidermal keratinocytes on a graftable collagen and chondroitin﷓6﷓sulfate substrate. Surgery 103:421﷓431.

10. Boyce ST, DJ Christianson and JF Hansbrough. 1988. Structure of a collagen﷓GAG dermal substitute optimized for cultured human epidermal keratinocytes. J Biomed Mat Res 22(10):939-957.

11. Boyce ST, MC Glafkides, TJ Foreman and JF Hansbrough. 1988. Reduced wound contraction after grafting of full thickness burns with a collagen and chondroitin﷓6﷓sulfate (GAG) dermal skin substitute and coverage with Biobrane. J Burn Care and Rehab 9(4):364-370.

12. Boyce ST, ML Cooper, TJ Foreman, BE Stompro and JF Hansbrough. 1989. Serum-free culture of human epidermal keratinocytes and applications to skin substitutes. Proceedings of the Second Bioscience Conference, Tokyo Metropolitan University, Tokyo, Japan, pp. 18-27.

13. Stompro BE, JF Hansbrough and ST Boyce. 1989. Attachment of peptide growth factors to implantable collagen. J Surg Res 46:413-421.

14. Hansbrough JF, ST Boyce, ML Cooper and TJ Foreman. 1989. Burn wound closure with cultured human keratinocytes and fibroblasts attached to a collagen-GAG substrate. J Amer Med Assn 262:2125-2130.

15. Boyce ST. 1990. Benchmarks: Alternative Methods in Toxicology. (Book review) J Invest Dermatol 94(1):155.

16. Cooper ML, ST Boyce, JF Hansbrough, TJ Foreman and D Frank. 1990. Cytotoxicity to cultured human keratinocytes (HK) of topical antimicrobial agents. J Surg Res 48(3):190-195.

17. Boyce ST, S Michel, M Regnier, B Shroot, R Schmidt. 1990. Reconstructed skin from cultured human keratinocytes and fibroblasts on a collagen-GAG biopolymer substrate. Skin Pharmacol 3(2):136-143.

18. Alexander JW, ST Boyce, GF Babcock, L Gionotti, MD Peck, DL Dunn, T Pyles, CP Childress and SK Ash. 1990. The process of microbial translocation. Ann Surg 212(4):496-512.

19. Kuroiwa K, JL Nelson, ST Boyce, JW Alexander, CK Ogle and S Inoue. 1991. Metabolic and immune effect of vitamin E supplementation after burn. J Parent Ent Nutri 15(1):22-26.

20. Sakai J, J Hung, G Zhu, R Cardell, S Boyce, and WW-Y Kao. 1991. Collagen metabolism during healing of lacerated rabbit corneas. Exptl Eye Res 52:237-244.

21. Cooper ML, RL Spielvogel, JF Hansbrough, ST Boyce and DH Frank. 1991. Reconstitution of the histological characteristics of a giant congenital nevomelanocytic nevus employing the athymic mouse and a cultured skin substitute. J Invest Dermatol 97(4):649-658.

22. Boyce ST, TJ Foreman, KB English, N Stayner, ML Cooper, S Sakabu and JF Hansbrough. 1991. Skin wound closure in athymic mice with cultured human cells, biopolymers, and growth factors. Surgery 110(5):866-876.

23. Boyce ST, B Stompro and JF Hansbrough. 1992. Biotinylation of implantable collagen for drug delivery. J Biomed Mat Res 26(4):547-553.

24. Boyce ST, P Furmanski, TJ Foreman, and JF Hansbrough. 1992. Absence of tumorigenicity in athymic mice by normal human epidermal keratinocytes after growth in serum free medium. Cancer Lett 62:141-147.

25. Boyce ST, D Greenhalgh, RJ Kagan, T Housinger, M Rieman, C Childress and GD Warden. 1993. Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers. Plast Reconstr Surg 91(4):632-641.

26. Boyce ST. 1993. Current usage, trends and research in skin grafts. SEOPF Tissue Banking Newsletter 3(1):1-4.

27. Boyce ST, EE Medrano, ZA Abdel-Malek, AP Supp, JM Dodick, JJ Nordlund and GD Warden. 1993. Pigmentation and inhibition of wound contraction by cultured skin substitutes with adult melanocytes after transplantation to athymic mice. J Invest Dermatol 100(4):360-365.

28. Hoath SB, R Tanaka and ST Boyce. 1993. Stratum corneum formation in the perinatal rat. J Invest Dermatol 100(4):400-406.

29. Boyce ST, JH James and ML Williams. 1993. Nutritional regulation of cultured analogues of human skin. J Toxicol - Cutan Ocular Toxicol 12(2):161-171.

30. Boyce ST and ML Williams. 1993. Lipid supplemented medium induces lamellar bodies and precursors of barrier lipids in cultured analogues of human skin. J Invest Dermatol 101(2):180-184.

31. Boyce ST and IA Holder. 1993. Selection of topical antimicrobial agents for cultured skin for burns by combined assessment of cellular cytotoxicity and antimicrobial activity. Plast Reconstr Surg 92(4):493-500.

32. Boyce ST, AP Supp, GD Warden and IA Holder. 1993. Attachment of an aminoglycoside, amikacin, to implantable collagen for local delivery in wounds. Antimicrobial Agents and Chemotherapy 37(9):1890-1895.

33. Porembka DT, A Kier, S Sehlhorst, S Boyce, JP Orlowski and K Davis. 1993. The pathophysiologic changes following bile aspiration in a porcine lung model. Chest 104(3):919-924.

34. Boyce ST. 1994. Epidermis as a secretory tissue. (editorial) J Invest Dermatol 102:8-10.

35. Boyce ST, IA Holder, AP Supp, DG Greenhalgh and GD Warden. 1994. Delivery and activity of antimicrobial drugs released from human fibrin sealant. J Burn Care Rehabil 15:251-255.

36. Holder IA, ST Boyce. 1994. Agar well diffusion assay testing of bacterial susceptibility to various antomicrobials in concentration non-toxic for human cells in culture. Burns 20(5):426-429.

37. Krueger GG, JR Morgan, CM Jorgensen, L Schmidt, HL Li, LT Li, ST Boyce, HS Wiley, J Kaplan and MJ Petersen. 1994. Genetically modified skin to treat disease: potentials and limitations. J Invest Dermatol 103(5):76s-84s.

38. Boyce ST, R Glatter, and WJ Kitzmiller. 1995. Treatment of chronic wounds with cultured cells and biopolymers: a pilot study. Wounds 7(1):24-29.

39. Boyce ST, AP Supp, MD Harriger, DG Greenhalgh and GD Warden. 1995. Topical nutrients promote engraftment and inhibit wound contraction of cultured skin substitutes in athymic mice. J Invest Dermatol 104(3):345-349.

40. Boyce ST, R Glatter, and WJ Kitzmiller. 1995. Case studies: Treatment of chronic wounds with cultured skin substitutes. Ostomy/Wound Management 41(2):26-33.

41. Harriger MD, GD Warden, DG Greenhalgh, RJ Kagan, and ST Boyce. 1995. Pigmentation and microanatomy of skin regenerated from composite grafts of cultured cells and biopolymers applied to full-thickness burn wounds. Transplantation 59(5):702-707.

42. Abdel-Malek ZA, VB Swope, C Akcali, MD Harriger, ST Boyce, K Urabe and VJ Hearing. 1995. Mitogenic and melanogenic stimulation of normal human melanocytes by melanotropic peptides. Proc Natl Acad Sci USA 92:1789-1793.

43. Boyce ST, GD Warden and IA Holder. 1995. Cytotoxicity testing of topical antimicrobial agents on human keratinocytes and fibroblasts for cultured skin grafts. J Burn Care Rehabil 16(2):97-103.

44. Farooqui JZ, E Robb, ST Boyce, GD Warden, and JJ Nordlund. 1995. Isolation of a unique melanogenic inhibitor from human skin xenografts: Initial in vitro and in vivo characterization. J Invest Dermatol 104:739-743.

45. Boyce ST, GD Warden and IA Holder. 1995. Non-cytotoxic combinations of topical antimicrobial agents for use with cultured skin. Antimicrobial Agents and Chemotherapy 39(6):1324-1328.

46. Goretsky MJ, M Breeden, G Pisarski, MD Harriger, ST Boyce, and DG Greenhalgh. 1995. Capillary morphogenesis during healing of full-thickness skin grafts: an ultrastructural study. Wound Repair Regen 3(2):213-220.

47. Boyce ST, MJ Goretsky, DG Greenhalgh, RJ Kagan, MT Rieman, and GD Warden. 1995. Comparative assessment of cultured skin substitutes and native skin autograft for treatment of full-thickness burns. Annals of Surgery 222(6):743-752.

48. Goretsky MJ, AP Supp, DG Greenhalgh, GD Warden, and ST Boyce. 1995. Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts in pediatric burn patients. Wound Repair Regen 3(4):419-425.

49. Goretsky MJ, MD Harriger, AP Supp, DG Greenhalgh, and ST Boyce. 1996. Expression of IL-1α, IL-6 and bFGF by cultured skin substitutes before and after grafting to full-thickness wounds in athymic mice. J Trauma 40(6):894-900.

50. Boyce ST, AP Supp, MD Harriger, WL Pickens, RR Wickett, and SB Hoath. 1996. Surface electrical capacitance as a non-invasive index of epidermal barrier in cultured skin substitutes in athymic mice. J Invest Dermatol 107(1):82-87.

51. Gennari R, JW Alexander, ST Boyce, N Lilly, GF Babcock, and M Cornaggia. 1996. Effects of the angiotensin converting enzyme inhibitor, Enalapril, on bacterial translocation after thermal injury and bacterial challenge. Shock 6(2):95-100.

52. Holder IA, and ST Boyce. 1996. Formulation of Aidealized@ topical antimicrobial mixtures for use with cultured skin substitutes. J Antimicrob Chemother 38:457-463.

53. Boyce ST. 1996. Cultured skin substitutes: a review. (Invited review) Tissue Engineering 2(4):255-266.

54. Boyce ST. 1997. Human Cell Culture Protocols. (Book review) J Invest Dermatol 108: 235.

55. Harriger MD, AP Supp, GD Warden, and ST Boyce. 1997. Glutaraldehyde crosslinking of collagen substrates inhibits degradation in skin substitutes grafted to athymic mice. J Biomed Mater Res 35:137-145.

56. Boyce ST, MD Harriger, AP Supp, GD Warden, IA Holder. 1997. Effective management of microbial contamination of cultured skin substitutes after grafting to athymic mice. Wound Repair Regen 5:191-197.

57. Swope VB, AP Supp, JR Cornelius, GF Babcock, and ST Boyce. 1997. Regulation of pigmentation in cultured skin substitutes by cytometric sorting of melanocytes and keratinocytes. J Invest Dermatol 109(3):289-295.

58. Harriger MD, AP Supp, VB Swope, and ST Boyce. 1997. Reduced engraftment and wound closure of cryopreserved cultured skin substitutes grafted to athymic mice. Cryobiology 35:132-142.

59. Vicanova J, M Ponec, J Bouwstra, VB Swope, ML Westbrook, MD Harriger, ST Boyce. 1997. Epidermal lipid metabolism of cultured skin substitutes during healing of full-thickness wounds in athymic mice. Wound Rep Regen 5:329-338.

60. Boyce ST. 1998. Cultured skin substitutes for treatment of burn wounds. Med Res Prog Shriners Hospitals for Children 1998:3-8.

61. Vicanova J, ST Boyce, MD Harriger, AM Weerheim, JA Bouwstra, and M Ponec. 1998. Stratum corneum lipid structure and composition in cultured skin substitutes is restored after grafting to athymic mice. J Invest Dermatol Symp Proc 3(2):114-120.

62. Wang Q, JJ Wang, ST Boyce, JE Fischer, P-O Hasselgren. 1998. Endotoxemia and IL-1β stimulate mucosal IL-6 production in different parts of the gastrointestinal tract. J Surg Res 76(1):27-31.

63. Can G, Z Abdel-Malek, PA Porter-Gill, P Gill, S Boyce, GA Grabowski, J Nordlund, and J Farooqui. 1998. Identification and sequencing of a putative variant of proopiomelanocortin in human epidermis and epidermal cell cultures. J Invest Dermatol 111:485-491 .

64. Boyce ST. 1998. Skin substitutes from cultured cells and collagen-GAG polymers. (Invited article) Medical and Biological Engineering and Computing 36:791-800.

65. Wang Q, TA Meyer, ST Boyce, JJ Wang, X Sun, G Tiao, JE Fischer, and PO Hasselgren. 1998. Endotoxemia in mice stimulates the production of complement C3 and serum amyloid A in mucosa of small intestine. Amer J Physiol 275:R1584-R1592.

66. Boyce ST, AP Supp, VB Swope, and GD Warden. 1999. Topical sulfamylon reduces engraftment of cultured skin substitutes. J Burn Care Rehabil 20(1):33-36.

67. Boyce ST. 1999. Cultured skin substitutes for burns. Med Res Prog Shriners Hospitals for Children 1999:55-56.

68. LePoole IC and ST Boyce. 1999. Keratinocytes suppress TGF-β1 expression by fibroblasts in cultured skin substitutes. Br J Dermatol 140(3):409-416.

69. Supp AP, RR Wickett, MD Harriger, VB Swope, SB Hoath, and ST Boyce. 1999. Incubation of cultured skin substitutes in reduced humidity stimulates epidermal barrier formation before and after grafting to athymic mice. Wound Repair Regen 7:226-237.

70. Holder IA and ST Boyce. 1999. Assessment of the potential for microbial resistance to topical use of multiple antimicrobial agents. Wound Repair Regen 7:238-243.

71. Boyce ST, RJ Kagan, NA Meyer, KP Yakuboff, and GD Warden. 1999. The 1999 Clinical Research Award. Cultured skin substitutes combined with IntegraJ Artificial Skin to replace native skin autograft and allograft for closure of full-thickness burns. J Burn Care Rehabil 20(6):453-461.

72. Boyce ST, AP Supp, RR Wickett, SB Hoath, and GD Warden. 2000. Assessment with the Dermal Torque Meter of skin pliability after treatment of burns with cultured skin substitutes. J Burn Care Rehabil 21:55-63.

73. Supp DM, AP Supp, S Bell, and ST Boyce. 2000. Enhanced vascularization of cultured skin substitutes genetically modified to overexpress Vascular Endothelial Growth Factor. J Invest Dermatol 114:5-13.

74. Supp DM, AP Supp, S Bell, VB Swope, JR Morgan, ST Boyce. 2000. Genetic modification of cultured skin substitutes by transduction of human keratinocytes and fibroblasts with Platelet Derived Growth Factor-A. Wound Repair Regen 8(1):26-35.

75. Swope VB, AP Supp, DG Greenhalgh, GD Warden and ST Boyce. 2001. Expression of insulin-like growth factor-I by cultured skin substitutes does not replace the physiologic requirement for insulin in vitro. J Invest Dermatol 116:650-657.

76. Boyce ST. 2001. Design principles for composition and performance of cultured skin substitutes. (Invited review article). Burns 27(5):523-533.

77. Boyce ST. 2001. Invited Discussion for: Morimoto et al, In vivo cultured skin composted of two-layer collagen sponges with preconfluent cells. Ann Plast Surg 47(1):74-82

78. Robb EC, N Beckmann, RT Plessinger, ST Boyce, GD Warden and RJ Kagan. 2001. The 2001 Lindberg Award. Storage media and temperature maintain normal anatomy of cadveric human skin for transplantation to full-thickness skin wounds. J Burn Care Rehabil 22(6):393-396.

79. Boyce ST, RJ Kagan, KP Yakuboff, NA Meyer, MT Rieman, and GD Warden. 2002. Cultured skin substitutes reduce donor skin harvesting in treatment of excised, full-thickness burns. Ann Surg 235(2):269-279.

80. Supp DM, and ST Boyce. 2002. Over-expression of Vascular Endothelial Growth Factor accelerates early vascularization and improves healing of genetically modified cultured skin substitutes. J Burn Care Rehabil 23:10-20.

81. Boyce ST and GD Warden. 2002. Principles and practices for cutaneous wound repair with cultured cells and biopolymers. (Invited article) Amer J Surg 183:445-456.

82. Boyce ST, VB Swope, AP Supp, and GD Warden. 2002. Vitamin C regulates keratinocyte viability, epidermal barrier and basement membrane in vitro, and reduces wound contraction after grafting of cultured skin substitutes. J Invest Dermatol 118(4):565-572.

83. Supp DM, KC Wilson-Landy, and ST Boyce. 2002. Human dermal microvascular endothelial cells form multicellular structures in cultured skin substitutes after transplantation to athymic mice. FASEB J 16:797-804.

84. Swope VB, AP Supp, GD Warden, and ST Boyce. 2002. Regulation of cutaneous pigmentation by titration of human melanocytes in cultured skin substitutes after grafting to athymic mice. Wound Repair Regen 10:378-386.

85. Holder IA, P Durkee, AP Supp, and ST Boyce. 2003. Assessment of a silver-coated dressing for potential use with skin grafts on excised burns. Burns 29:445-448.

86. Boyce ST. 2004. Fabrication, quality assurance and assessment of cultured skin substitutes for treatment of skin wounds. Biomed Eng J 20:107-112.

87. Passaretti D, D Billmire, R Kagan, J Corcoran, and S Boyce. 2004. Autologous cultured skin substitutes conserve donor skin autograft in elective treatment of giant congenital melanocytic nevus. Plast Reconstr Surg 114(6):1523-1528.

88. Supp DM, AC Karpinski, and ST Boyce. 2004. VEGF overexpression increases vascularization by murine, but not human, endothelial cells in cultured skin substitutes grafted to athymic mice. J Burn Care Rehabil 25:337-345.

89. Supp DM, AC Karpinski, and ST Boyce. 2004. Expression of defensins HBD-1, -2, -3, in cultured keratinocytes and cultured skin substitutes. Burns 30(7):643-648.

90. Supp AP, AN Neely, DM Supp, GD Warden, and ST Boyce. 2005. Evaluation of cytotoxicity in vitro of Acticoat® burn dressing for management of microbial contamination in cultured skin substitutes grafted to athymic mice. J Burn Care Rehabil 26(3):238-246.

91. Swope VB and ST Boyce. 2005. Differential expression of matrix metalloproteinase-1 in vitro corresponds to tissue morphogenesis and quality assurance of cultured skin substitutes. J Surg Res 128:79-86.

92. Boyce ST, RJ Kagan, DG Greenhalgh, KP Yakuboff, P Warner, T Palmieri and GD Warden. 2005. Cultured skin substitutes reduce donor skin harvesting in treatment of excised, full-thickness burns. J Trauma, in press.

93. Smiley AK, JM Klingenberg, BJ Aronow, ST Boyce, and DM Supp. 2005. Microarray analysis of gene expression in cultured skin substitutes compared with native human skin. J Invest Dermatol, in press.

94. Smiley AK, JM Klingenberg, ST Boyce, and DM Supp. 2005. Keratin expression in cultured skin substitutes suggests that the hyperproliferative phenotype in vitro is normalized after grafting. Burns, in press.

95. Barai ND, AP Supp, ST Boyce, GB Kasting and MO Visscher. 2005. Improvement of barrier properties in cultured skin substitutes after grafting onto athymic mice. Skin Pharmacology and Physiology, in press.

96. Boyce ST, BA Anderson, and HL Rilo. 2005. Quantitative assay for quality assurance of human cells for clinical transplantation. Cell Transplantation, in press.

97. Boyce ST, RR Wickett, SB Hoath, and M Visscher. 2005. Multi-variate analysis of biophysical properties of human skin measured quantitatively with non-invasive instruments. In preparation.

98. Boyce ST, AP Supp, MD Harriger, VB Swope, and GD Warden. 2005. Fibroblasts in cultured skin substitutes regulate development of basement membrane and epidermal barrier. In preparation.


ABSTRACTS:

1. Boyce S and E Fifkova. 1980. Synaptic changes in the visual cortex of monocularly deprived hooded rats. Society for Neuroscience Abstracts 6:492.

2. Boyce ST and RG Ham. 1981. Control of differentiation, frozen storage, and serum﷓free culture of human epidermal keratinocytes. In Vitro 17:239.

3. Boyce ST and GD Shipley. 1983. Comparison of mitogenic activities of PDGF, FGF and EGF on human diploid fibroblasts and Swiss 3T3 cells in serum﷓free medium. In Vitro 18:305.

4. Fritz KA, SR Ryan, ST Boyce, WL Weston and DA Norris. 1983. Bullous pemphigoid or pemphigus antisera fail to induce epidermal cell cytotoxicity using an improved in vitro technique. Clinical Res 31:146A.

5. Ryan SR, DA Norris, KA Fritz, ST Boyce and WL Weston. 1983. Comparison of mechanisms of epidermal cytotoxicity in vitro. Clinical Research 31:150A.

6. Boyce ST and JF Hansbrough. 1984. Growth requirements of cultured human epidermal cells on a collagen-glycosaminoglycan membrane. Proc Amer Burn Assn 16:107.

7. Huff JC, DA Norris, MG Cary, RM Kissinger, ST Boyce and WL Weston. 1984. Induction of HLA﷓DR antigens in cultured human keratinocytes by stimulated peripheral blood mononuclear cells. Clin Res 32(2):591A.

8. Huff JC, DA Norris, MG Cary, RM Kissinger, ST Boyce and WL Weston. 1984. Study of HLA﷓DR antigens in human keratinocytes in culture. Clinical Research 32(1):139A.

9. Boyce ST, MC Glafkides, TJ Foreman and JF Hansbrough. 1986. Reduced wound contraction after excision and grafting of full thickness burns with a collagen and chondroitin﷓6﷓sulfate dermal skin replacement and coverage with Biobrane. Proc Amer Burn Assn 18:30.

10. Boyce ST, DJ Perkins and JF Hansbrough. 1986. Regulation of the structural pore size of a collagen and chondroitin﷓6﷓sulfate dermal skin replacement. Proc 12th Ann Soc for Biomater 9:92.

11. Boyce ST, E Roberts and JF Hansbrough. 1987. Confluent cultures of normal human epidermal keratinocytes (HK) exhibit reduced growth potential compared to subconfluent cultures. Proc Amer Burn Assn 19:30.

12. Boyce ST, S Sakabu, TJ Foreman and JF Hansbrough. 1987. Athymic (nude) mice as a model for evaluation of wound contraction after grafting of materials for human skin replacement. Proc Amer Burn Assn 19:78.

13. Boyce ST, DJ Christianson and JF Hansbrough. 1987. Concentration of starting materials affects the structural pore size and thickness of a collagen and chondroitin﷓6﷓sulfate dermal skin replacement. Proc 13th Ann Soc for Biomater 10:119.

14. Boyce ST and JF Hansbrough. 1987. Biological attachment, growth and differentiation in vitro of human epidermal keratinocytes onto a graftable collagen and chondroitin﷓6﷓sulfate membrane. J Invest Derm 88(4):479.

15. Boyce ST, TJ Foreman, P Furmanski and JF Hansbrough. 1987. Absence of tumor formation in athymic mice by normal human epidermal keratinocytes after growth in culture. Proc Amer Assn Cancer Res 28:30.

16. Boyce ST and JF Hansbrough. 1987. In vivo testing of an autograft substitute. Program of the 1987 Geneva Congress on Burns, Abstr 6B02.

17. Boyce ST and JF Hansbrough. 1988. Cell cycle analysis vs time of cultured keratinocyte population subsets after growth on collagen﷓GAG substrates. Proc Amer Burn Assn 20:14.

18. Stompro BE, JF Hansbrough and ST Boyce. 1988. Incorporation of basic fibroblast growth factor, and of epidermal growth factor into implantable collagen. Proc Assn Acad Surgery 22:139.

19. Cooper M, S Boyce, T Foreman, D Frank and J Hansbrough. 1989. Case report: Rapid formation of anchoring fibrils on a re-epithelialized full-thickness burn after treatment with dermal-epidermal skin substitutes. Proc Amer Burn Assn 21:17.

20. Boyce S, M Cooper, T Foreman and J Hansbrough. 1989. Human recombinant heparin binding growth factor 2 can replace epidermal growth factor for clonal growth of human epidermal keratinocytes in biochemically defined medium. Proc Amer Burn Assn 21:164.

21. Boyce S, T Foreman, K English, M Cooper and J Hansbrough. 1989. Increased acceptance and decreased wound contraction on athymic mice of cultured skin substitutes after growth in serum-free media. Proc Amer Burn Assn 21:165.

22. Cooper M, J Hansbrough, D Frank, T Foreman and S Boyce. 1989. Cytotoxicity to cultured human keratinocytes (HK) of topical antimicrobial agents. Proc Amer Burn Assn 21:166.

23. Cooper M, D Frank, JF Hansbrough, TJ Foreman and ST Boyce. 1989. Methods for evaluating the viability and epithelial thickness of a cultured skin substitute. Proc 39th Meeting of the Calif Soc Plast Surgeons.

24. Boyce ST, BE Stompro and JF Hansbrough. 1989. Biotinylation of implantable collagen for attachment of biologically active molecules in wound treatment. Proc 15th Ann Soc for Biomater 12:196.

25. Boyce ST, P Davis, C-H Fang and JW Alexander. 1990. Serum-free culture conditions for simultaneous transplantation of human epidermal keratinocytes and dermal fibroblasts. Proc Amer Burn Assn 22:3.

26. Boyce ST, CP Childress, C-H Fang, P Davis, A Supp and JW Alexander. 1990. Progressive development in vitro of basement membrane precursors by cultured human keratinocytes on collagen-GAG substrates. Proc Amer Burn Assn 22:9.

27. Boyce ST, V Hegde, A Supp and JW Alexander. 1990. Tensile strength analysis of a dermal-epidermal cultures skin substitute. Proc Amer Burn Assn 22:111.

28. Cooper ML, J Hansbrough, T Foreman, J Laxer, S Sakabu and S Boyce. 1990. Early formation of basement membrane with an autologous dermal-epidemral composite cultured skin substitute: a clinical series. Proc Amer Burn Assn 22:1.

29. Cooper ML, S Sakabu, T Foreman, J Hansbrough and S Boyce. 1990. In vitro initiation of basement membrane with a dermal-epidermal composite skin substitute. Proc Amer Burn Assn 22:46p.

30. Boyce ST, VC Hegde, AP Supp and JW Alexander. 1990. Device for failure load and strain analyses of cultured skin substitutes. Proc 16th Ann Soc for Biomater 13:115.

31. Alexander JW, ST Boyce, GF Babcock, DL Dunn and M.L. Peck. 1990. The process of microbial translocation. Proc Amer Surg Assn.

32. Boyce ST, V Hegde, SB Hoath, MM Donnelly, JM Dodick, CP Childress and AP Supp. 1990. Initial characterization of stress analysis and keratinization of lifted vs submerged dermal-epidermal cultured skin substitutes. J Invest Dermatol 94(4):509.

33. Cooper M, R Spielvogel, JF Hansbrough, ST Boyce, TJ Foreman and D Frank. 1990. An animal model for the study of giant congenital nevomelanocytic nevi. J Invest Dermatol 94(4):515.

34. Boyce ST, SB Hoath, V Hegde and MM Donnelly. 1990. Characterization of stress analysis and keratinization of lifted versus submerged dermal-epidermal cultured skin substitutes for biomedical applications. Ohio Symp on Advances in Biomaterials Science and Engineering.

35. Hoath SB, ST Boyce and MM Donnelly. 1990. Preparation of intact sheets of mammalian stratum corneum (SC) of determinate thickness for biomaterials research. Ohio Symp on Advances in Biomaterials Science and Engineering.

36. Boyce ST, DG Greenhalgh, TA Housinger, RJ Kagan, M Rieman, CP Childress and GD Warden. 1991. Histologic and ultrastructural analyses of full-thickness burns closed with cultured cell-biopolymer skin substitutes. Proc of the American Burn Association 23:8.

37. Boyce ST and ML Williams. 1991. Free fatty acids and vitamin E induce lamellar bodies and precursors of barrier lipids in lifted dermal-epidermal cultured skin substitutes. J Invest Dermatol 96(4):620A.

38. Boyce ST and ML Williams. 1991. Lipid supplemented culture medium induces lamellar bodies and precursors of barrier lipids in lifted dermal-epidermal cultured skin substitutes. Gordon Research Conference on Barrier Function of Mammalian Skin.

39. Boyce ST, EE Medrano, ZA Abdel-Malek and JJ Nordlund. 1991. Pigmentation of cultured epithelium by human melanocytes in a composite skin substitute model. J Cell Biol 115(3)pt2:357a.

40. Boyce ST and IA Holder. 1992. Assessment of topical antimicrobial agents for use with cultured skin grafts on burn wounds. Proc Amer Burn Assn 24:43.

41. Boyce ST, AP Supp and IA Holder. 1992. Attachment of biotinylated amikacin to implantable collagen for cultured skin substitutes. Proc Fourth World Biomaterials Congress:488.

42. Boyce ST and ML Williams. 1992. Growth and biochemical characterization of human keratinocytes without glucose and acetate in submerged, lifted and low humidity culture. J Invest Derm 98(4):638.

43. Hoath SB, R Tanaka and ST Boyce. 1992. Rate of stratum corneum formation in the perinatal rat. J Invest Derm 98(4):584.

44. Hoath SB, R Tanaka and ST Boyce. 1992. Regulation of terminal differentiation (programmed cell death) in perinatal rat epidermis: effect of age and epidermal growth factor (EGF). Ped Res 31(4)pt2:44a(abst 248).

45. Boyce ST, EE Medrano, ZA Abdel-Malek, A Supp and JJ Nordlund. 1992. Pigmentation and wound contraction in athymic mice of cultured skin with melanocytes incubated in selective media. Proc Wound Healing Soc 2:32.

46. Boyce ST, EE Medrano, ZA Abdel-Malek and JJ Nordlund. 1992. Pigmentation of cultured epithelium by human melanocytes after grafting to athymic mice. Pan Amer Pigment Cell Res 4:A664.

47. Morse MA, ST Boyce, CC Daugherty and MM Ziegler. 1992. Human biliary epithelial cells for in vitro studies of biliary atresia and other cholangiopathies. In Vitro Cell Devel Biol 28(3):157A.

48. Boyce ST. 1992. Nutritional regulation of epidermal barrier in reconstructed human skin. Pharmacol Res 9(10, supplement):S-216.

49. Boyce ST, AP Supp, JM Dodick, CP Childress, EE Medrano, JJ Nordlund and GD Warden. 1993. Topical nutrients and incubation media provide conditions for complete engraftment and inhibition of wound contraction by cultured skin grafts with melanocytes applied to athymic mice. Proc Amer Burn Assn 25:3.

50. Boyce ST and IA Holder. 1993. Cytotoxicity testing of human keratinocytes and fibroblasts to topical antimicrobial agents for use with cultured skin grafts. Proc Amer Burn Assn 25:7.

51. Boyce ST, IA Holder, AP Supp and DG Greenhalgh. 1993. Delivery and activity of antimicrobial drugs released from human fibrin sealant. Proc Amer Burn Assn 25:99.

52. Boyce ST, SB Hoath, R Wickett, MD Harriger and ML Williams. 1993. Loss of requirement for exogenous epidermal growth factor by cultured analogues of normal human skin. J Invest Dermatol 100(4):579.

53. Harriger MD and ST Boyce. 1993. Human melanocytes transfer pigment to keratinocytes in a cultured skin substitute after grafting to athymic mice. Proc Microscopy Soc Amer, 51st Annual Meeting:400-401.

54. Boyce ST, MD Harriger, AP Supp, JM Dodick, DG Greenhalgh and GD Warden. 1993. Topical nutrients promote complete engraftment and inhibit wound contraction of composite cultured skin in athymic mice. Wound Repair and Regen 1(2):98.

55. Boyce ST, GD Warden and IA Holder. 1994. Non-toxic combinations of topical antimicrobial agents for use with cultured skin. Proc Amer Burn Assn 26:103.

56. Boyce ST, AP Supp, GD Warden and SB Hoath. 1994. Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes in vitro and after grafting to athymic mice. Proc Amer Burn Assn 26:124.

57. Harriger MD, GD Warden and ST Boyce. 1994. Microanatomy of skin regenerated from composite skin substitutes after grafting to excised, full-thickness burns. Proc Amer Burn Assn 26:123.

58. Holder IA and ST Boyce. 1994. Agar well antimicrobial diffusion assay testing of bacterial susceptibility/resistance to concentrations of antimicrobials non-toxic for cells in culture. Proc Amer Burn Assn 26:230.

59. Boyce ST, AP Supp, WL Pickens, SB Hoath. 1994. Surface electrical capacitance as a non-invasive measure of epidermal barrier of cultured skin substitutes in vitro and in vivo. J Invest Dermatol 102(4):537.

60. Boyce ST, MD Harriger, AP Supp and GD Warden. 1994. Glutaraldehyde crosslinking of collagen implants in vitro inhibits biodegradation after grafting of cell-collagen composites to athymic mice. Wound Repair and Regen 2(1):73.

61. Goretsky MJ, M Breeden, G Pisarski, MD Harriger, ST Boyce, DG Greenhalgh. 1994. Time course of capillary surface ultrastructure during healing of full-thickness skin grafts. Wound Repair and Regen 2(1):78.

62. Reid J, Lipscomb GG, S Boyce. 1994. Development of a novel collagen membrane for use as an artificial skin support. Proc of the 1994 meeting of the Materials Research Society.

63. Boyce ST, CQ Sheeler and IA Holder. 1994. Assay of cytotoxicity of antimicrobial agents by correlation of keratinocyte numbers with optical density of crystal violet. In Vitro Cell Devel Biol 30A(3,ptII):84-85.

64. Supp AP, SB Hoath, WL Pickens and ST Boyce. 1994. Time course of development of epidermal barrier in cultured skin substitutes as determined by surface electrical capacitance. Proc 10th International Symposium on Bioengineering and the Skin.

65. Glatter R, ST Boyce, and WJ Kitzmiller. 1994. Treatment of chronic cutaneous ulcers with a cultured skin substitute. Ohio Biotech 94 Symposium.

66. Boyce ST, MJ Goretsky, DG Greenhalgh, RJ Kagan, M Rieman, and GD Warden. 1995. Comparative assessment of early outcome of cultured skin substitutes and skin autograft for treatment of excised full-thickness burns. Proc Amer Burn Assn 27:126.

67. Boyce ST, AP Supp, M Rieman, GF Babcock, and GD Warden. 1995. ELISA assay for antibodies to bovine collagen in sera from burn patients grafted with cultured skin substitutes. Proc Amer Burn Assn 27:125.

68. Boyce ST, MD Harriger, AP Supp, VB Swope, and GD Warden. 1995. Optimized dressing protocols for engraftment of cultured skin substitutes after grafting to athymic mice. Proc Amer Burn Assn 27:149.

69. Holder IA and ST Boyce. 1995. Broad spectrum antimicrobial mixtures for clinical use with cultured skin autografts: in vitro studies. Proc Amer Burn Assn 27:152.

70. Goretsky MJ, AP Supp, DG Greenhalgh, GD Warden, and ST Boyce. 1995. Surface electrical capacitance as an index of epidermal barrier properties of composite skin substitutes and skin autografts in pediatric burn patients. Wound Repair Regen 3(1):77.

71. Boyce ST, AP Supp, MD Harriger, VB Swope, and GD Warden. 1995. Effective management of microbial contamination of cultured skin substitutes on athymic mice with formulations of non-cytotoxic antimicrobial agents. Wound Repair Regen 3(2):83.

72. Boyce ST, VB Swope, and GD Warden. 1995. Responses of keratinocyte proliferation to insulin-like growth factor-1 (IGF-1) and human growth hormone in serum-free medium. J Invest Dermatol 104(4):641.

73. Boyce ST, J Vicanova, MD Harriger, AP Supp, VB Swope, NA Lilly, and M Ponec. 1995. Biochemical and biophysical analyses of epidermal barrier formation in cultured skin substitutes grafted to athymic mice. Gordon Research Conference on Barrier Function of Mammalian Skin.

74. Goretsky MJ, MD Harriger, AP Supp, DG Greenhalgh, and ST Boyce. 1995. In vivo production of IL-1, IL-6, and bFGF by cultured skin substitutes engrafted on full-thickness wounds in athymic mice. Proceedings of the 1995 meeting of the American Association of Surgery of Trauma.

75. Supp AP, RR Wickett, SB Hoath, GD Warden, and ST Boyce. 1996. Reduced humidity during incubation of skin substitutes in vitro accelerates epidermal barrier formation after grafting to athymic mice. Proc Amer Burn Assn Vol 28:178.
76. Harriger MD, GD Warden, and ST Boyce. 1996. Molecular identification of allogeneic keratinocytes for banking of cultured skin substitutes. Proc Amer Burn Assn Vol 28:179.

77. Boyce ST, JB Wiener, GD Warden, and IA Holder. 1996. Non-cytotoxic antimicrobials for topical use on cultured skin substitutes. Proc Amer Burn Assn Vol 28:174.

78. Boyce ST, J Vicanova, J Bouwstra, MD Harriger, AP Supp, VB Swope, and M Ponec. 1996. Biochemical and biophysical analyses of epidermal barrier formation in cultured skin substitutes grafted to athymic mice. Journal of Investigative Dermatology 106(4):916.

79. Swope VB, JR Cornelius, GF Babcock, and ST Boyce. 1996. Flow cytometric separation of epidermal keratinocytes and melanocytes. J Invest Dermatol 106(4):882.

80. Farooqui J, G Can, S Boyce, and J Nordlund. 1996. Expression of proopiomelanocortin and its peptides in human epidermis and epidermal cells (melanocytes & keratinocytes). J Invest Dermatol 106(4):883.

81. Boyce ST, VB Swope, AP Supp, MD Harriger, and GD Warden. 1996. Cryopreservation of cultured skin substitutes reduces engraftment in athymic mice. Wound Repair Regen 4(1):A145.

82. Hoath SB, RR Wickett, and ST Boyce. 1996. Electrical measurements and skin barrier function in cultured and neonatal skin. Proceedings of International Society for Bioengineering and the Skin.

83. Can G, Z Abdel-Malek, S Boyce, J Nordlund, and J Farooqui. 1996. Regulation of ACTH, α-MSH and β-endorphin expression in cultures of normal human melanocytes and keratinocytes. Pigment Cells Res suppl 5:244a.

84. Robb E, MD Harriger, ML Westbrook, ST Boyce, and RJ Kagan. 1996. Viability testing of banked allograft skin. 20th Annual meeting of the American Association of Tissue Banks; poster S-8.

85. Vicanova J, M Ponec, MD Harriger, and ST Boyce. 1996. Restoration of epidermal lipid composition in cultured skin substitutes grafted to athymic mice. J Invest Dermatol 107(3):519 (Abstract #427).

86. Supp AP, RR Wickett, and ST Boyce. 1996. Reduced humidity stimulates barrier development in skin substitutes as measured by electrical properties. Proceedings of the 20th Anniversary Symposium of the International Society for Bioengineering and the Skin, poster #16.

87. Swope VB, and ST Boyce. 1996. Clonal growth response of human keratinocytes to KGF and IGF-1. Molec Biol Cell 7:140a.

88. Boyce ST, AP Supp, VB Swope, MD Harriger, and GD Warden. 1997. Sulfamylon reduces engraftment of cultured skin substitutes in athymic mice. J Burn Care Rehabil 18(1, pt 3):S181.

89. Boyce ST, AP Supp, RR Wickett, and GD Warden. 1997. Biophysical instrumentation for non-invasive assessment of skin wounds. J Burn Care Rehabil 18(1, pt 3):S198.

90. Farooqui JZ, C Gong, S Boyce, E Robb, G Warden, and J Nordlund. 1997. Proopiomelanocortin and its peptides are expressed in cultured skin substitutes grafted on athymic mice. J Burn Care Rehabil 18(1, pt 3):S186.

91. Boyce ST, AP Supp, and VB Swope. 1997. Optimized epidermal barrier in vitro and wound healing in vivo of cultured skin substitutes by media formulations. J Invest Dermatol 108(4):568.

92. Swope VB, AP Supp, and ST Boyce. 1997. Insulin-free medium supports epidermal barrier formation in cultured skin substitutes. J Invest Dermatol 108(4):637.

93. Swope VB, JZ Farooqui, G Can, and ST Boyce. 1997. Expression of insulin-like growth factor-1 by cultured skin substitutes. Wound Rep Regen 5(1):A125.

94. Farooqui J, G Can, S Boyce, G Warden, and J Nordlund. 1997. Effect of cell-cell interaction on the production of proopiomelanocortin peptides in vitro and in vivo. Pigment Cell Res 10:121a.

95. Supp AP, RR Wickett, and ST Boyce. 1997. Reduced humidity stimulates epidermal barrier formation in cultured skin substitutes in vitro. Proc of the Society for Cosmetic Chemists annual meeting 3:11.

96. Robb EC, N Bechmann, RT Plessinger, ST Boyce, and RJ Kagan. 1997. A comparison of changed vs unchanged media for viability testing of banked allograft skin. 1997 Annual Meeting of the American Association of Tissue Banks.

97. Boyce ST, VB Swope, AP Supp, and GD Warden. 1997. Vitamin C promotes epidermal barrier and DNA synthesis in keratinocytes of cultured skin substitutes. Molec Biol Cell 8:339a.

98. LePoole IC and ST Boyce. 1997. Keratinocytes reduce total TGFβ-1 levels in cultured skin substitutes. Molec Biol Cell 8:340a.

99. Boyce ST, AP Supp, VB Swope, and GD Warden. 1998. Xeric stress of cultured skin substitutes in vitro increases tolerance to desiccation after grafting to athymic mice. J Burn Care Rehab 19(1,pt 2):S234.

100. Boyce ST, AP Supp, and GD Warden. 1998. Instrumental assessment of visco-elastic properties in skin after treatment of full-thickness burns with cultured skin substitutes. J Burn Care Rehab 19(1,pt 2):S245.

101. Farooqui J, S Boyce, E Robb, G Warden, and J Nordlund. 1998. Identification and characterization of a proopiomelanocortin mRNA variant in human skin. J Burn Care Rehab 19(1,pt 2):S232.

102. Holder IA and ST Boyce. 1998. A topical antimicrobial mixture for cultured skin autografts: resistance development consideration. J Burn Care Rehab 19(1,pt 2):S217.

103. Robb EC, N Bechmann, RT Plessinger, ST Boyce, RJ Kagan, and GD Warden. 1998. The enhanced effect of media on banked allograft skin. J Burn Care Rehab 19(1,pt 2):S231.

104. Boyce ST, RJ Kagan, KP Yakuboff, and GD Warden. 1998. Cultured skin substitutes increase utilization of donor sites in treatment of full-thickness wounds. J Invest Dermatol 110(4):679.

105. Farooqui J, A Budev, S Boyce, and J Nordlund. 1998. Expression of a putative proopiomelanocortin mRNA variant in human epidermis and epidermal cells in culture. J Invest Dermatol 110(4):529.

106. Boyce ST, RJ Kagan, KP Yakuboff, and GD Warden. 1998. Cultured skin substitutes increase utilization of donor sites in treatment of full-thickness burns. Wound Repair and Regeneration 6(3):A242.

107. Boyce ST, AP Supp, VB Swope, GD Warden. 1998. Vitamin C stimulates epidermal barrier and reduces wound contraction in healing of skin substitutes. Wound Repair and Regeneration 6(3):A249.

108. Supp DM, AP Supp, JR Morgan, and ST Boyce. 1998. Genetic modification of cultured skin substitutes by transduction of human keratinocytes with PDGF-A. Wound Repair and Regeneration 6(3):A265.

109. MacLennan SE, WJ Kitzmiller, ST Boyce, and SB Hoath. 1998. The use of cultured skin substitutes for full-thickness skin loss in the neonate. 1998 meeting of Plastic and Reconstructive Surgeons.

110. Suzuki I, A Tada, V Swope, S Boyce, and Z Abdel-Malek. 1998. Regulation of MC1 receptor expression in human melanocytes. Pigment Cell Res 11(3):171.

111. Boyce ST, RJ Kagan, NA Meyer, KP Yakuboff, and GD Warden. 1999. Cultured skin substitutes combined with IntegraJ for closure of full-thickness burns. J Burn Care Rehabil 20:S196.

112. Boyce ST, VB Swope, and GD Warden. 1999. Fibroblasts in cultured skin substitutes stimulate epidermal barrier and increase cellular viability. J Burn Care Rehabil 20:S197.

113. Boyce ST, VB Swope, AP Supp, and GD Warden. 1999. Vitamin C stimulates viability and barrier in vitro, and promotes earlier wound closure with cultured skin substitutes. J Burn Care Rehabil 20:S224.

114. Boyce ST, RJ Kagan, NA Meyer, KP Yakuboff, and GD Warden. 1999. Cultured skin substitutes reduce requirements for donor skin autograft in treatment of full-thickness burns. J Burn Care Rehabil 20:S228.

115. Supp DM, AP Supp, S Bell, and ST Boyce. 1999. Enhanced expression of Vascular Endothelial Growth Factor (VEGF) in human keratinocytes by retroviral transduction. J Burn Care Rehabil 20:S223.

116. Farooqui JZ, A Budev, ST Boyce, EC Robb, and GD Warden. 1999. Expression of proopiomelanocortin converting enzymes prohormone convertases 1 and 2 in normal human skin. J Burn Care Rehabil 20:S224.

117. Robb EC, N Beckmann, RT Plessinger, ST Boyce, GD Warden, and RJ Kagan. 1999. A comparison of various storage methods and conditions for viability testing of banked allograft skin. J Burn Care Rehabil 20:S228.

118. Boyce ST, AP Supp, and GD Warden. 1999. Cultured skin substitutes with fibroblasts express basement membrane antigens and ultrastructure in vitro. J Invest Dermatol 112(4):586.

119. Supp DM, AP Supp, S Bell, and ST Boyce. 1999. Expression of biologically-active Vascular Endothelial Growth Factor in cultured skin substitutes. J Invest Dermatol 112(4):591.

120. Moro O, MB Davis, V Swope, S Boyce, K Dixon, and Z Abdel-Malek. 1999. Human melanocytes are less sensitive than keratinocytes or fibroblasts to the cytotoxic and genotoxic effects of ultraviolet radiation and arsenic. Pigment Cell Res 7:38.

121. Boyce ST, VB Swope, and GD Warden. 1999. Expression of protein and message for IGF-1 in keratinocytes of cultured skin substitutes. Wound Repair Regen 7(4):A290.

122. Supp DM, AP Supp, S Bell, and ST Boyce. 1999. Enhanced vascularization of cultured skin substitutes genetically modified to overexpress vascular endothelial growth factor. Wound Repair Regen 7(4):A321.

123. Holder IA and ST Boyce. 1999. Clinical management of microbial contamination in cultured skin substitutes for full-thickness burns. Wound Repair Regen 7(4):A257.

124. Boyce ST, RJ Kagan, NA Meyer, KP Yakuboff, and GD Warden. 2000. Autologous cultured skin substitutes reduce donor skin harvesting in grafting of extensive, full-thickness burns. J Burn Care Rehabil 21:S190.

125. Boyce ST, RJ Kagan, JF Corcoran, and GD Warden. 2000. Autologous cultured skin substitutes conserve donor autograft for elective reconstruction of burn scar and congenital giant nevus. J Burn Care Rehabil 21:S222.

126. Boyce ST, AP Supp, and GD Warden. 2000. Fibroblasts in cultured skin substitutes reduce wound contraction and facilitate epithelial function after grafting to athymic mice. J Burn Care Rehabil 21:S211.

127. Boyce ST, AP Supp, and GD Warden. 2000. Assessment by laser Doppler flowmetry of blood flow in cultured skin substitutes after grafting to athymic mice. J Burn Care Rehabil 21:S212.

128. Supp DM, AP Supp, S Bell, JR Morgan, GD Warden, and ST Boyce. 2000. Overexpression of platelet-derived growth factor-A in genetically modified cultured skin substitutes. J Burn Care Rehabil 21:S218.

129. Boyce ST, KC Wilson and GD Warden. 2000. Endothelial cells form multicellular structures in cultured skin substitutes in vitro. J Invest Dermatol 114(4):809.

130. Swope VB, AP Supp and ST Boyce. 2000. Pigmentation of cultured skin substitutes in vitro by addition of cultured melanocytes. J Invest Dermatol 114(4):818.

131. Supp AP, VB Swope and ST Boyce. 2000. Vitamin C promotes expression of basement membrane antigens and histiotypic epithelium in cultured skin substitutes. J Invest Dermatol 114(4):809.

132. Pereira E, K Dixon, V Swope, S Boyce, and Z Abdel-Malek. 2000. Multiplicity of the stress-induced pathways in epidermal cells. J Invest Dermatol 114(4):829.

133. Supp DM, and ST Boyce. 2000. Enhanced vascularization of cultured skin substitutes genetically modified to over-express vascular endothelial growth factor. Proc of NIH Conference on Cutaneous Gene Therapy.

134. Koval NM, and ST Boyce. 2000. Tube formation by human microvascular endothelial cells after addition to cultured skin substitutes and transplantation to athymic mice. University of Cincinnati Medical Student Research Conference.

135. Barai ND, AP Supp, ST Boyce, GB Kasting. 2001. Barrier properties of cultured skin substitutes. AAPS Pharm Sci 3(3):abstract #1780.

136. Warden GD, M Rieman, L Fowler, L Golden, and ST Boyce. 2001. Surface electrical capacitance as a quantitative index of donor site healing. J Burn Care Rehabil 22(2):S75.

137. Boyce ST, RJ Kagan, KP Yakuboff, NA Meyer and GD Warden. 2001. Comparative assessment of qualitative outcome after treatment of excised burns with cultured skin substitutes and split-thickness autograft. J Burn Care Rehabil 22(2):S114.

138. Boyce ST, VB Swope, AP Supp, and GD Warden. 2001. Regulation of pigmentation of cultured skin substitutes grafted to athymic mice by controlled addition of melanocytes. J Burn Care Rehabil 22(2):S50.

139. Boyce ST, AP Supp, and GD Warden. 2001. Correlation of surface electrical capacitance with surface pH in cultured skin substitutes in vitro. J Burn Care Rehabil 22(2):S118.

140. Supp DM, GD Warden, and ST Boyce. 2001. Improved performance of cultured skin substitutes genetically engineered to over express vascular endothelial cell growth factor. J Burn Care Rehabil 22(2):S74.

141. Robb EC, N Beckmann, RT Plessinger, ST Boyce, GD Warden and RJ Kagan. 2001. The 2001 Lindberg Award: Storage media and temperature maintain normal anatomy of cadveric human skin for transplantation to full-thickness skin wounds. J Burn Care Rehabil 22(2):S130.

142. Swope VB, AP Supp, GD Warden, and ST Boyce. 2001. Restoration of pigmentation in cutaneous wounds by titration of melanocytes into cultured skin substitutes grafted to athymic mice. J Invest Dermatol 117(2):513.

143. Boyce ST, AP Supp, and GD Warden. 2002. Cytotoxicity testing of Acticoat7 Burn Dressing with cultured skin substitutes in vitro and after grafting to athymic mice. J Burn Care Rehabil 23(2):S131.

144. Supp AP, GB Kasting, GD Warden, and ST Boyce. 2002. Assessment of barrier properties in cultured skin substitutes by surface electrical capacitance and percutaneous penetration of 3H2O. J Burn Care Rehabil 23(2):S130.

145. Supp DM, GD Warden, and ST Boyce. 2002. Human microvascular endothelial cells form vascular analogs in cultured skin substitutes grafted to athymic mice. J Burn Care Rehabil 23(2):S90 .

146. Passaretti D, D Billmire, J Corcoran, R Kagan, and S Boyce. 2002. Successful treatment of giant congenital nevi with autologous cultured skin substitutes. Ohio Valley Society of Plastic Surgeons.

147. Greenhalgh DG, J Sanders, MB Lawless, TL Palmieri, and ST Boyce. 2003. Transcontinental transport of cultured skin substitutes to cover massive burns. J Burn Care Rehabil 24(2):S127.

148. Boyce ST, AP Supp, H Gunt, GD Warden, and GB Kasting. 2003. Assessment of barrier properties of cultured skin substitutes before and after grafting to athymic mice. J Burn Care Rehabil 24(2):S124.

149. Supp DM, AC Karpinski, GD Warden, and ST Boyce. 2003. VEGF-modified keratinocytes do not enhance proliferation of transplanted human endothelial cell in cultured skin substitutes grafted to athymic mice. J Burn Care Rehabil 24(2):S126.

150. Robb EC, N Beckmann, M Alf, RT Plessinger, ST Boyce, GD Warden, and RJ Kagan. 2003. A comparison of 4EC, room temperature, and 37EC storage conditions on the anatomy of cadaveric human skin. J Burn Care Rehabil 24(2):S88.

151. Boyce ST, AP Supp, VB Swope and GD Warden. 2003. Cultured skin substitutes in vitro express multiple phenotypes of normal human skin. Mol Biol Cell, Abstract 709A.

152. Boyce S, R Kagan, K Yakuboff, P Warner, T Schuermann, M Kleiner, C Lloyd, J Miller, and G Warden. 2004. Quantitative and qualitative assessment of cultured skin substitutes and split-thickness skin autograft in treatment of excised, full-thickness burns. J Burn Care Rehabil 25(2):S66.

153. Boyce S, A Supp, M Reed, and G Warden. 2004. Assessment of skin pliability after grafting of cultured skin substitutes and split-thickness autografts over Integra Artificial Skin or cadaveric allograft. J Burn Care Rehabil 25(2):S172.

154. Supp AP, AN Neely, DM Supp, GD Warden, and ST Boyce. 2004. Evaluation of Acticoat Burn Dressing for management of microbial contamination in cultured skin substitutes grafted to athymic mice. J Burn Care Rehabil 25(2):S172.

155. Boyce ST, VB Swope, AP Supp, and GD Warden. 2004. Comparative assessment of methods for primary culture of epidermal keratinocytes for cultured skin substitutes. J Burn Care Rehabil 25(2):S173.

156. Supp DM, A Karpinski, GD Warden, and ST Boyce. 2004. Expression of human beta defensin in cultured keratinocytes and cultured skin substitutes. J Burn Care Rehabil 25(2):S169.

157. Koizumi T, EC Robb, N Beckmann, M Alf, RT Plessinger, ST Boyce, Y Iwanaga, H Rilo, GD Warden, RJ Kagan. 2004. Prolonging human skin viability using 95% oxygen-enriched perfluorocarbon. J Burn Care Rehabil 25(2):S80.

158. Supp DM, AC Karpinski, and ST Boyce. 2004. Keratin expression in cultured skin substitutes suggests a hyperprolifertive state that is normalized after grafting to athymic mice. J Invest Dermatol 122(3): A74.

159. Boyce ST, MA Reed, RJ Kagan, KP Yakuboff, PA Warner, and GD Warden. 2005. Quantitative assessment of growth of cultured skin substitutes for 2-7 years following application in pediatric patients treated for extensive, full-thickness burns. J Burn Care Rehabil 26(2):S167.

160. Kagan RJ, ST Boyce, and R Gillespie. 2005. The use of skin grafts and skin substitutes: results of a survey with proposed revisions to current procedural terminology (CPT). J Burn Care Rehabil 26(2):S75.

161. ST Boyce, VB Swope, AP Supp, and RJ Kagan. 2005. Regulation of pigment expression in vitro in co-cultures of human fibroblasts, melanocytes and keratinocytes. J Burn Care Rehabil 26(2):S167.

162. Boyce ST, AP Supp, MA Reed, and RJ Kagan. 2005. Quantitative assessment of skin pliability after treatment of full-thickness burns with cultured skin substitutes and split-thickness autografts combined with Integra Artificial SkinÒ or cadaveric allograft. J Burn Care Rehabil 26(2):S168.

163. Supp DM, A Smiley, J Klingenberg, S Boyce, R Kagan. 2005. Microarray of gene expression in cultured skin substitutes. J Burn Care Rehabil 26(2):S103.

TRAINEES: Dates Position/Institution

Michael Glafkides, M.D. Jul-Dec 86 Surg Res: Univ of Calif San Diego

Mark Sherman, M.D. May-Sep 86 Med Student: Univ of Calif San Diego

Charles Richardson, M.D. May-Sep 86 Med Student: Univ of Calif San Diego

Sandra Weiss, B.S. Sep 87-Jun 88 Undergrad: Univ of Calif San Diego

Brett Stompro, M.D. Jan 88-Jun 88 Surg Res: Univ of Calif San Diego

Matthew Cooper, M.D. Jul 88-Dec 88 Surg Res: Univ of Calif San Diego

Jennifer Reid, M.S. Sep 92-Nov 93 Masters: Univ of Cincinnati, Engineering

Michael Goretsky, M.D. Jul 93-Jun 95 Surg Res: Univ of Cincinnati

M. Dana Harriger, Ph.D. Jan 94-Jun 96 Res Fellow: Shriners Hospital for Children

Andrew P. Supp, B.S. Jan 94-Dec 97 Masters: Univ of Cincinnati, Pharmacy

Cameron Q. Sheeler, B.S. Sep 94-Dec 94 Ph.D.: Univ of Cincinnati, Cell Biology

Rajeet Pannu, B.S. Oct 95 Masters: Univ of Alberta, Cell Biology

I. Caroline LePoole, Ph.D. Jan-Jun 97 Res Fellow: Univ of Cincinnati, Surgery

Dorothy M. Supp, Ph.D. Jul 97-Dec 99 Res Fellow: Shriners Hospitals for Children

Nikkiya Koval, B.S. Jun 00-Aug 00 Med Student: University of Cincinnati

Mark Kleiner, B.S. Sep 00-Dec 04 Masters: Univ of Cincinnati, Chemistry

Saja Hamed, B.S. Sep 00-Jun 04 Ph.D.: Univ of Cincinnati, Pharmacy

Namrata Barai, M.S. Sep 01-Jun 05 Ph.D.: Univ of Cincinnati, Pharmacy

Balaji Kalyanaraman, M.D. Sep 02-Jun 07 Ph.D.: Univ of Cincinnati, Biomed Eng

Heather Powell, Ph.D. Dec 04-Jun 07 Res Fellow; Shriners Hospitals for Children

Alexis Armour, M.D. Oct-Dec 05 Res Fellow; Shriners Hospitals for Children

REVIEWER FOR:

Editorial Board: Journal of Burn Care and Rehabilitation

Journals: Annals of Plastic Surgery
Archives of Dermatology
Biomaterials
Biotechnology and Bioengineering
Experimental Cell Research
In Vitro Cellular and Developmental Biology
Journal of Burn Care and Rehabilitation
Journal of Cellular Physiology
Journal of Clinical Investigation
Journal of Investigative Dermatology
Journal of Dermatological Science
Journal of Trauma
Laboratory Investigation
Proceedings of the National Academy of the Sciences
Skin Pharmacology
Tissue Engineering
Transplantation
Trends in Biotechnology

Grants: Edison BioTechnology Center
Johns Hopkins University Center for Alternatives to Animal Testing
NIH/Yale Skin Research Center
NIH/DRG Technology and Applied Sciences (SBIR/STTR); 10/96
NIH/CSR Technology and Applied Sciences (SBIR/STTR); 07/98
NIH/CSR Surgery, Anesthesiology & Trauma Rev Grp;
02/00; 02/01; 02/03; 10/04
NIH/CSR Biomed Res Partnerships & Grants (BRPs, BRGs); 03/00; 07/01
NIH/CSR Centers of Excellence in Complex Biomedical Systems Res 03/02
NIH/CSR Biomedical and Tissue Engineering 03/02, 11/02
US Army Medical Research and Development Command 11/03
Medical Research Council of the United Kingdom 3/05
Wound Healing Foundation Research Fellowships 3/05
NIH Special Emphasis for GCRC review 05/05

RESEARCH FUNDING HISTORY:

ACTIVE: TOTAL DIRECT COSTS AWARDED

Sponsor: NIH; RO1 GM 50509
Title: Mechanisms of Wound Healing with Cultured Human Skin
Position: Principal Investigator, 35%
Dates: 07/01/02 - 06/30/06 $ 960,000

Sponsor: US Food & Drug Administration; FD-R-000672-07
Title: Cultured Skin Substitutes for Closure of Burn Wounds
Position: Principal Investigator, 35%
Dates: 10/01/03 - 09/30/06 $ 801,865

Sponsor: Shriners of North America; SHC #8670
Title: Skin Repair with Cultured Cells and Biopolymers
Position: Principal Investigator, 10%
Dates: 01/01/03 - 12/31/05 $ 825,000

Sponsor: Shriners of North America; SHC #8450
Title: Special Shared Facility - Microscopy & Imaging
Position: Principal Investigator, 10%
Dates: 01/01/02 - 12/31/04 $ 260,000

Sponsor: Shriners of North America; SHC #8680
Title: Genetic Engineering of Cultured Skin Substitutes to
Improve Wound Healing
Position: Co-Investigator, 10%
Dates: 01/01/03 - 12/31/05 $ 450,000

Sponsor: NIH Career Development Award
Title: Development of Vascular Networks for Transplantation
Position: Advisor to Jennifer Marler, M.D. (no salary)
Dates: 07/01/03 - 06/30/08 $ 350,000

Sponsor: Shriners of North America; SHC (new)
Title: Expression of β-Defensins in Cultured Skin Substitutes
Position: Co-Investigator, 10%
Dates: 01/01/04 - 12/31/06 $ 450,000

PAST:

Sponsor: US Food & Drug Administration; FD-R-000672-07
Title: Cultured Skin Substitutes for Closure of Burn Wounds
Position: Principal Investigator, 35%
Dates: 10/01/00 - 09/30/03 $ 591,263

Sponsor: NIH GM 08478-01
Title: Burn/Trauma Research Training Grant
Position: Co-Program Director, no salary; PI, JS Solomkin, MD
Dates: 07/01/98 - 06/30/03 $ 675,000

Sponsor: Shriners of North America; SHC #8670
Title: Skin Repair with Cultured Cells and Biopolymers
Position: Principal Investigator, 10%
Dates: 01/01/02 - 12/31/02 $150,000

Sponsor: Shriners of North America; SHC #8680
Title: Genetic Engineering of Cultured Skin Substitutes to
Improve Wound Healing
Position: Co-Investigator, 10%
Dates: 01/01/00 - 12/31/02 $ 320,000

Sponsor: NIH; RO1 GM 50509
Title: Mechanisms of Wound Healing with Cultured Human Skin
Position: Principal Investigator, 35%
Dates: 07/01/98 - 06/30/02 $ 748,934

Sponsor: Shriners of North America; SHC #8450/#8460
Title: Special Shared Facility - Microscopy & Imaging
Position: Principal Investigator, 10%
Dates: 01/01/99 - 12/31/01 $250,000

Sponsor: Shriners of North America; SHC #8670
Title: Skin Repair with Cultured Cells and Biopolymers
Position: Principal Investigator, 30%
Dates: 01/01/99 - 12/31/01 $ 450,000

Sponsor: US Food & Drug Administration; FD-R-000672-07
Title: Cultured Skin Substitutes for Closure of Burn Wounds
Position: Principal Investigator, 35%
Dates: 10/01/97 - 09/30/00 $ 587,231

Sponsor: NIH; AR42832
Title: Expression and Regulation of Proopiomelanocortin in Human Melanocytes
Position: Co-investigator, 6%; PI, JZ Farooqui, PhD
Dates: 06/01/95 - 05/31/99 $ 600,000

Sponsor: Shriners of North America; SHC #8670
Title: Skin Repair with Cultured Cells and Biopolymers
Position: Principal Investigator, 30%
Dates: 01/01/96 - 12/31/98 $ 450,000

Sponsor: Shriners of North America; SHC #8450/#8460
Title: Special Shared Facility - Microscopy & Imaging
Position: Principal Investigator, 10%
Dates: 01/01/98 - 12/31/98 $ 105,000

Sponsor: NIH; RO1 GM 50509-01-04
Title: Mechanisms of Wound Healing with Cultured Human Skin
Position: Principal Investigator, 35%
Dates: 01/01/94 - 06/30/98 $ 640,000

Sponsor: Shriners of North America; SHC #8460
Title: Special Shared Facility - Electron Microscopy
Position: Principal Investigator, no salary
Dates: 01/01/95 - 12/31/97 $ 150,000

Sponsor: Shriners of North America; SHC #8450
Title: Special Shared Facility - Histology
Position: Principal Investigator, 10%
Dates: 01/01/95 - 12/31/97 $ 180,000

Sponsor: US Food & Drug Administration; FD-R-000672
Title: Cultured Skin Substitutes for Closure of Burn Wounds
Position: Principal Investigator, 16%
Dates: 08/01/94 - 07/31/97 $ 290,000

Sponsor: NIH; AI 12936-16
Title: Host Defense Mechanisms: Control of Surgical Infection
Position: Co-Investigator, 4%; PI, JW Alexander, MD, DSc
Dates: 09/01/93 - 08/31/97 $ 1,200,000

Sponsor: NIH; HD 28528-01A1
Title: Use of Genetically Modified Skin to Treat Disease
Position: Consultant, no salary; PI, GG Krueger, MD
Dates: 06/01/92 - 05/31/97 $2,993,789

Sponsor: Shriners of North America; SHCC #8670
Title: Skin Regeneration with Cultured Cells and Biopolymers
Position: Principal Investigator, 35%
Dates: 01/01/94 - 12/31/95 $ 250,000

Sponsor: The Jewish Hospital #499-273402
Title: Treatment of Chronic Cutaneous Ulcers with a Cultured Skin Substitute
Position: Co-Investigator, no salary; PI, WJ Kitzmiller, MD
Dates: 09/01/93 - 08/31/95 $ 46,000

Sponsor: University of Cincinnati Research Challenge Program
Title: Presence of Proopiomelanocortin and Its Peptides in Human Epidermis
Position: Co-Investigator, no salary; PI, J Farooqui, PhD
Dates: 07/01/94- 12/31/94 $ 42,596

Sponsor: US Food & Drug Administration; FD-R-000672-01
Title: Cultured Skin Substitutes for Closure of Burn Wounds
Position: Principal Investigator, 10%
Dates: 04/01/91 - 07/31/94 $ 270,000

Sponsor: NIH; GM 31754-09
Title: Mechanisms of Neutrophil Dysfunction in Burns and Trauma
Position: Co-Investigator, 5%; PI, JS Solomkin, MD
Dates: 04/01/91 - 03/31/94 $ 450,000

Sponsor: NIH-SBIR Phase I
Title: In Vitro Model for Dermal Irritation
Position: Consultant, no salary; MatTek Corporation
Dates: 09/01/93 - 02/28/94 $ 50,000

Sponsor: The Jewish Hospital #499-273401
Title: Treatment of Chronic Cutaneous Ulcers with a Cultured Skin Substitute
Position: Co-Investigator, no salary; PI, WJ Kitzmiller, MD
Dates: 03/01/93 - 08/31/93 $ 27,650

Sponsor: NIH; AI 12936-13
Title: Host Defense Mechanisms: Control of Surgical Infection
Position: Co-Investigator, 5%; PI, JW Alexander, MD, ScD
Dates: 09/01/90 - 08/31/93 $ 1,191,000

Sponsor: Shriners of North America; SHCC #15893
Title: Permanent Skin Substitutes for Closure of Burn Wounds
Position: Principal Investigator, 50%
Dates: 01/01/92 - 12/31/92 $ 90,000

Sponsor: Shriners of North America; SHCC #15837/#15846
Title: Special Shared Facility - Electron Microscopy
Position: Principal Investigator, 25%
Dates: 01/01/89 - 12/31/94 $ 475,000

Sponsor: Shriners of North America; SHCC #15893
Title: Permanent Skin Substitutes for Closure of Burn Wounds
Position: Principal Investigator, 50%
Dates: 01/01/89 - 12/31/91 $ 605,000

Sponsor: NIH GM08241
Title: Burn/Trauma Research Training Grant
Position: Co-Program Director; PI, JF Hansbrough, MD
Dates: 07/01/88 - 06/30/89 $ 70,000

Sponsor: Clonetics Corporation
Title: In Vivo Testing of Cultured Skin Substitutes
Position: Co-investigator; PI, JF Hansbrough, MD
Dates: 04/01/88 - 08/31/88 $ 67,500

Sponsor: NIH GM35068
Title: Living Prosthetic Skin
Position: Co-investigator; PI, JF Hansbrough, MD
Dates: 02/01/85 - 07/31/88 $ 873,255

Sponsor: NIH GM31465
Title: Living Prosthetic Skin
Position: Co-investigator; PI, JF Hansbrough, MD
Dates: 03/01/83 - 01/31/85 $ 85,322

Sponsor: US Army Research and Development Command
Title: Differentiated Properties of Cultured Human Keratinocytes
Position: Principal Investigator
Dates: 07/01/82 - 12/31/82 $ 10,000

Sponsor: Minnesota Mining and Manufacturing
Title: Scholarship for Skin Research
Position: Principal Investigator
Dates: 01/01/82 - 01/01/83 $ 3,000

UC/Shriners LECTURES:

Date Event Title

1. 01 Feb 89 SBI/UC Res seminar Cultured skin substitutes

2. 28 Sep 89 SBI nursing inservice Clinical care of cultured skin

3. 22 Nov 89 Dept of Surgery res sem Burn wound closure with cultured skin

4. 31 Jan 90 Plastic Surgery Wound closure with cultured epithelium
Grand Rounds

5. 03 Apr 90 Dept of Dermatology Cultured and reconstructed epithelium
Basic science lecture for grafting of wounds

6. 29 Jun 90 Tiss Cult Assn Cultured skin from human cells and
regional conference biopolymers

7. 18 Jul 90 Pediatric Surgery Wound closure with cultured skin
Grand Rounds

8. 15 Nov 91 Chemical Engineering Engineering of skin tissue from
Center of Excellence cultured cells and biopolymers

9. 04 Mar 92 Plastic Surgery Clinical use of cultured skin for burns
Grand Rounds

10. 26 Oct 93 Dermatology Regeneration of epidermal barrier and
Basic Science pigmentation after grafting of wounds with cultured skin

11. 07 May 94 Surgery Clinical treatment of wounds with cultured Grand Rounds skin substitutes

12. 22 Jun 94 Nursing Inservice Clinical protocols for treatment of burns with cultured skin substitutes

13. 28 Feb 95 Dermatology Wound treatment with cultured skin Basic Science substitutes

14. 15 Apr 96 College of Pharmacy Wound healing in burns

15. 23 Jul 96 Dermatology Wound treatment with cultured skin Basic Science substitutes

16. 09 Sep 98 Plastic Surgery Alternative materials for closure of skin
Grand Rounds wounds

17. 07 Jan 99 Dermatology Clinical applications of cultured skin
Clinical Research substitute

18. 18 Nov 98 College of Pharmacy Skin substitutes for wound treatment

19. 26 May 99 Shriners Burns Hospital Cultured skin combined with IntegraJ
Nursing Research Council

20. 08 Jul 99 Dermatology Skin substitutes
Clinical Research

21. 29 Sep 99 Surgery Treatment of cutaneous wounds with
Grand Rounds cultured skin substitutes

22. 25 Jul 00 Shriners Burns Hospital Engineering of skin substitutes
Nursing Research Council

23. 07 Nov 00 College of Engineering Wound repair by tissue engineering
Engineering Grad Students

24. 03 May 01 College of Arts & Sciences Cutaneous wound repair by tissue
Introduction to Biomaterials engineering

25. 19 Dec 01 Surgery Wound closure with cultured skin
Grand Rounds substitutes

26. 19 Feb 02 Surgery Skin repair with cultured cells and
Visiting Professor Seminar biopolymers

27. 13 May 02 College of Engineering Cutaneous wound repair by tissue
Engineering Grad Students engineering

28. 15 May 02 Surgery Cultured skin substitutes for burn treatment
Visiting Professor Seminar

29. 13 Jun 02 Biomedical Engineering Tissue engineering of human skin
Intercollegiate Seminar

14. 09 Oct 03 CCHRF; Devel & Disease The skin

15. 30 Oct 03 Biomedical Engineering Tissue engineering of human skin

16. 30 Nov 04 Biomedical Engineering Tissue engineering of human skin

INTRAMURAL COMMITTEES AND SERVICE:

1992-1994 Director, Shriner=s Burns Institute Research Conference

1994 Dean=s Committee on the Future of Basic Sciences at the UC College of Medicine

1996 Mediator for College of Medicine in faculty grievance

1999 Dean=s Task Force on the Future of the Department of Dermatology

1999-2005 Univ of Cincinnati Dept of Surgery Committee for Appointment, Reappointment, Promotion and Tenure

2002-2004 Univ of Cincinnati Dept of Biomedical Engineering, Graduate Curriculum Committee

OTHER SERVICE AND PUBLIC RELATIONS:

1. Re-commissioned the Corbett Laboratories for Electron Microscopy, January, 1989.

2. Associated Press article; October, 1989 - clinical use of cultured skin.

3. Life Choices TV video; July, 1990 - innovative burn treatments.

4. Cincinnati Post article; November, 1990 - cultured skin for burns.

5. Ohio Magazine; Spring 1993 - Ohio biotechnology update.

6. TV video for Orlando, Florida NBC affiliate; Spring, 1993 - "Cultured skin for burns".

7. Dermatology Times Magazine; Spring, 1993 - "Topical nutrients promote engraftment of cultured skin".

8. Nagoya News 1 (Nagoya, Japan); November, 1995 - ABanking of cultured skin for wound treatment@.

9. Channel 9 News (Cincinnati, OH); July, 1997 - AClinical advantages of cultured skin for burns@.

10. M.D. News Magazine (Cincinnati, OH); April, 1998 - ATreatment of burns with cultured skin substitutes@.

11. UC Research Magazine (Cincinnati, OH); August, 1998 - AWound treatment with cultured skin substitutes@.

12. Time-Life Books for Children (Alexandria, VA); November, 1998 - ASkin grafts made in the laboratory@.

13. Cincinnati Enquirer; December, 1998 - ASkin from the lab saves an arm@.

14. Between Us Magazine; July, 1999 - ACultured skin substitutes to treat pediatric burns@

15. National Public Radio, Science News; July, 2000 - ACloning skin to heal burns@

16. The Cincinnati Post; Front page; August, 2000 - AGrowing skin for Atarah@

17. Findings; NIGMS news magazine; February, 2001 - AArtificial skin heals burn wounds@

18. BioOhio; Business development magazine; September, 2001 - ASpotlight on biotechnology@

19. Public Broadcasting System; Kid=s Health Series; November, 2001 - ACultured skin for burns@

20. Shriners Hospitals 80th Anniversary Video; December, 2001 - AAdvances in burn treatment@

21. FDA Consumer Magazine; May, 2002 - AFuture products for burn care@

22. Cleveland Plain Dealer Newspaper; January, 2003 - ABiotechnology for burns@

23. Technology Review; February, 2003 - AEngineered skin with blood vessels@

24. Channel 4 News (Orlando, FL); July, 2003 – “Advanced treatments for burns”

25. The Cincinnati Enquirer; November, 2003 – “Burn research receives $1M gift”

26. Forest Hills Journal; November, 2004 – “Local scientist delivers keynote in Australia”